Transmucosal transport of tobramycin incorporated in solid lipid nanoparticles (SLN) after duodenal administration to rats. Part II - Tissue distribution

Tobramycin-loaded solid lipid nanoparticles (SLN) were prepared and adminis tered by duodenal and intravenous (i.v.) routes to rats and the tissue dist ributions were determined successively at fixed times (30 min, 4 h and 24 h ) and compared to those of the tobramycin solution after i.v. administratio n. The tissue distribution between tobramycin-loaded SLN administered duodenal ly and i.v. was different.. A marked difference between tobramycin-loaded S LN administered duodenally and tobramycin solution administered i.v. was al so evidenced. In particular, the amounts of tobramycin in the kidneys after tobramycin-loaded SLN administration either duodenally or i.v. were lower than after administration of i.v. solution. Tobramycin-loaded SLN were able to pass across the blood-brain barrier in rats to a greater extent after i .v. injection than after duodenal administration. (C) 2001 Academic Press.