Vl. Shapovalov et al., Effect of fluoride anions on gramicidin photoinactivation sensitized by sulfonated aluminum phthalocyanines, PHOTOCHEM P, 74(1), 2001, pp. 1-7
Interaction of potent photodynamic agents, sulfonated aluminum phthalocyani
nes (AlPcSn where n is a number of sulfonic groups), with biological membra
nes was studied here using model systems: sensitized photoinactivation of g
ramicidin channels in planar lipid bilayers and adsorption on lipid monolay
ers, Fluoride anions known to form complexes with aluminum were found to in
hibit both the adsorption of aluminum phthalocyanines on lipid monolayers,
as measured with a Langmuir trough by surface pressure and surface potentia
l changes, and photodynamic efficacy of the dyes, as studied by gramicidin
channel photoinactivation, The similar effects were caused by the alkaliniz
ation of the medium. Fluoride anions appeared to be much more effective in
the case of AlPcS4 as compared to AlPcS3. The suppression of the photodynam
ic potency of aluminum phthalocyanines was attributed to desorption of the
dyes from lipid bilayers induced by fluoride or hydroxyl ions. With AlPcS4
an enhancement of the dye aggregation leading to a decrease in the sensitiz
ing activity was probably involved in the fluoride effect as revealed by ab
sorption and fluorescence spectral measurements. Capillary electrophoresis
was employed to understand the mechanism of the dye desorption, The results
of these experiments indicated that the reduction in the membrane affinity
was associated with an increase in the negative charge of the dye molecule
s due to the binding of fluoride or hydroxyl ions.