Protein-RNA interactions in the subunits of human nuclear RNase P

Authors
Jiang, TJ Guerrier-Takada, C Altman, S
Citation
Tj. Jiang et al., Protein-RNA interactions in the subunits of human nuclear RNase P, RNA, 7(7), 2001, pp. 937-941
Citations number
18
Categorie Soggetti
Biochemistry & Biophysics
Journal title
RNA-A PUBLICATION OF THE RNA SOCIETY
ISSN journal
13558382 → ACNP
Volume
7
Issue
7
Year of publication
2001
Pages
937 - 941
Database
ISI
SICI code
1355-8382(200107)7:7<937:PIITSO>2.0.ZU;2-6
Abstract
A yeast three-hybrid system was employed to analyze interactions in vivo be tween H1 RNA, the RNA subunit of human nuclear RNase P, and eight of the pr otein subunits of the enzyme. The genetic analysis indicates that subunits Rpp21, Rpp29, Rpp30, and Rpp38 interact directly with H1 RNA. The results o f direct UV crosslinking studies of the purified RNase P holoenzyme confirm the results of the three-hybrid assay.