The relationship between the platelet storage lesion (PSL) and programmed c
ell death (apoptosis) is poorly understood. Nevertheless, there is some exp
erimental evidence that platelets contain most of the components of the apo
ptosis machinery and both the apoptotic process and the PSL lead to platele
t activation and microvesiculation with expression of phosphatidyl serine (
PS) on the outer layer of cell membrane, a hallmark of all nucleated cells.
The PS exposure is believed to contribute to the development of inflammato
ry or immunomodulation process, to the regulation of haemostatic balance an
d the ultimate clearance of dead or fragmented cells from the circulation.
While there is no doubt that apoptosis, as a form of genetically encoded pr
ogrammed cell death in nucleated cells, is triggered by several signalling
stimuli at the nuclear level, there is some doubt as to whether platelets,
as enucleated cells have retained the memory of the "parental" megakaryocyt
es for apoptosis or whether platelet mitochondrial DNA has a major role in
both the apoptotic process and the PSL. The storage lesion occurs during pr
ocessing and storage subsequent to mechanical trauma, hypoxic conditions or
exposure to cold. In this brief report some observational evidence is prov
ided in support of the notion that the PSL and apoptosis may be related to
each other. despite the Fact that, in contrast to the 'parental' megakarocy
te, the platelets appear to survive upon stimulation with a high concentrat
ion of protein kinase inhibitors such as staurosporine (STS), in the presen
ce of cycloheximide (CHX) which inhibit protein synthesis. This is a model
which is often used to regulate the level of survival signals. The possible
relevance of platelet microvesiculation to transfusion practice is briefly
discussed. (C) 2001 Elsevier Science Ltd. All rights reserved.