Immunoelectron microscopy on material retrieved from paraffin: Accurate sampling on the basis of stained paraffin sections

Citation
Kp. Dingemans et M. Ramkema, Immunoelectron microscopy on material retrieved from paraffin: Accurate sampling on the basis of stained paraffin sections, ULTRA PATH, 25(3), 2001, pp. 201-206
Citations number
9
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
ULTRASTRUCTURAL PATHOLOGY
ISSN journal
01913123 → ACNP
Volume
25
Issue
3
Year of publication
2001
Pages
201 - 206
Database
ISI
SICI code
0191-3123(200105/06)25:3<201:IMOMRF>2.0.ZU;2-P
Abstract
As a rule, immunoelectron microscopy (immuno-EM) is performed on fresh mate rial processed according to specialized methods (e.g., freezing or embeddin g in hydrophilic resins). Paraffin-embedded tissue has only occasionally be en used as a source of material for immuno-EM; this was usually as a last r esort, when no fresh material was available. The authors used archival form alin-fixed. paraffin-embedded basal cell carcinomas for studying the fine-s tructural distribution of the cell surface molecule CD44 and its variants, as well as some other antigens. The results demonstrate, firstly, that para ffin-embedded material is far more suitable for immuno-EM than frequently a ssumed and, secondly, that the use of paraffin-embedded material enables hi ghly accurate sampling on the basis of immunohistochemically or conventiona lly stained light microscopic sections.