Aa. Siddiqui et al., STRONGYLOIDES-STERCORALIS - IDENTIFICATION OF ANTIGENS IN NATURAL HUMAN INFECTIONS FROM ENDEMIC AREAS OF THE UNITED-STATES, Parasitology research, 83(7), 1997, pp. 655-658
Using Western-blot analysis, we identified eight immunodominant antige
ns (apparent molecular weights 96, 86, 75, 56, 41, 32, 28, and 26 kDa)
of Strongyloides stercoralis in natural human infections. For this st
udy, 78 individual serum samples were obtained from S. stercoralis-inf
ected patients residing in endemic areas of the United States. Poly A(
+) RNA was translated in vitro in the rabbit-reticulocyte lysate syste
m. The newly synthesized translation products were immune-precipitated
with S. stercoralis human infection sera. All eight of the identified
antigens were detected in the immunoprecipitates. The potential of th
ese antigens as targets for immunodiagnosis is also discussed.