STRONGYLOIDES-STERCORALIS - IDENTIFICATION OF ANTIGENS IN NATURAL HUMAN INFECTIONS FROM ENDEMIC AREAS OF THE UNITED-STATES

Citation
Aa. Siddiqui et al., STRONGYLOIDES-STERCORALIS - IDENTIFICATION OF ANTIGENS IN NATURAL HUMAN INFECTIONS FROM ENDEMIC AREAS OF THE UNITED-STATES, Parasitology research, 83(7), 1997, pp. 655-658
Citations number
23
Categorie Soggetti
Parasitiology
Journal title
ISSN journal
09320113
Volume
83
Issue
7
Year of publication
1997
Pages
655 - 658
Database
ISI
SICI code
0932-0113(1997)83:7<655:S-IOAI>2.0.ZU;2-H
Abstract
Using Western-blot analysis, we identified eight immunodominant antige ns (apparent molecular weights 96, 86, 75, 56, 41, 32, 28, and 26 kDa) of Strongyloides stercoralis in natural human infections. For this st udy, 78 individual serum samples were obtained from S. stercoralis-inf ected patients residing in endemic areas of the United States. Poly A( +) RNA was translated in vitro in the rabbit-reticulocyte lysate syste m. The newly synthesized translation products were immune-precipitated with S. stercoralis human infection sera. All eight of the identified antigens were detected in the immunoprecipitates. The potential of th ese antigens as targets for immunodiagnosis is also discussed.