A NEW METHOD FOR ISOLATION AND DIFFERENTIATION OF NATIVE ENTAMOEBA-HISTOLYTICA AND ENTAMOEBA-DISPAR CYSTS FROM FECAL SAMPLES

Authors
WALDERICH B MULLER L BRACHA R KNOBLOCH J BURCHARD GD
Citation
B. Walderich et al., A NEW METHOD FOR ISOLATION AND DIFFERENTIATION OF NATIVE ENTAMOEBA-HISTOLYTICA AND ENTAMOEBA-DISPAR CYSTS FROM FECAL SAMPLES, Parasitology research, 83(7), 1997, pp. 719-721
Citations number
11
Categorie Soggetti
Parasitiology
Journal title
Parasitology research → ACNP
ISSN journal
09320113
Volume
83
Issue
7
Year of publication
1997
Pages
719 - 721
Database
ISI
SICI code
0932-0113(1997)83:7<719:ANMFIA>2.0.ZU;2-9
Abstract
A new method for the purification of protozoan cysts from feces was es tablished, allowing the isolation of native cysts. The procedure consi sts of two sucrose-density gradients and enzymatic digestion of cellul ose particles by cellulase and can be accomplished in a few hours. The cyst fractions were differentiated into Entamoeba histolytica and E. dispar using the DNA probes P145 and B133 in a dot-blot test.