Enzyme modified microband electrodes: cross-talk effects and their elimination

One microband of an array of four microband electrodes (1 mm long and 25 mu m wide with a 25 mum gap) was modified with glucose oxidase by direct elect rochemically assisted immobilisation, giving a stable microbiosensor with a n apparent Michaelis-Menten constant of 12 mM and an i(max) of 80 nA. Cross -talk effects on the adjacent microbands were studied and three different m ethods for their elimination were tested; the most efficient one involved c atalase deposition on the adjacent microband. Under these conditions, the m aximum response at the unmodified microbands was in the worst case about 3% compared with the response of the modified microband. This approach has th e potential to fabricate a multianalyte microbiosensor.