Enzyme modified microband electrodes: cross-talk effects and their elimination

Citation
M. Quinto et al., Enzyme modified microband electrodes: cross-talk effects and their elimination, ANALYST, 126(7), 2001, pp. 1068-1072
Citations number
33
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYST
ISSN journal
00032654 → ACNP
Volume
126
Issue
7
Year of publication
2001
Pages
1068 - 1072
Database
ISI
SICI code
0003-2654(2001)126:7<1068:EMMECE>2.0.ZU;2-4
Abstract
One microband of an array of four microband electrodes (1 mm long and 25 mu m wide with a 25 mum gap) was modified with glucose oxidase by direct elect rochemically assisted immobilisation, giving a stable microbiosensor with a n apparent Michaelis-Menten constant of 12 mM and an i(max) of 80 nA. Cross -talk effects on the adjacent microbands were studied and three different m ethods for their elimination were tested; the most efficient one involved c atalase deposition on the adjacent microband. Under these conditions, the m aximum response at the unmodified microbands was in the worst case about 3% compared with the response of the modified microband. This approach has th e potential to fabricate a multianalyte microbiosensor.