Physical mapping of CSF2RA, ANT3 and STS on the pseudoautosomal region of bovine chromosome X

The pseudoautosomal region (PAR) of bovine chromosome X (BTA X) has a parti cularly low representation of genes and markers, making comparative gene ma pping in this region difficult. We describe the localization of three genes , colony-stimulating factor 2 receptor alpha (CSF2RA), ADP/ATP translocase 3 (ANT3) and steroid sulphatase (STS) on PAR of BTA X using a 5000 rad whol e-genome radiation hybrid panel, The relationship of these genes to a numbe r of previously mapped simple sequence repeat (microsatellite) markers is d etermined by physical and radiation hybrid mapping methods. The resulting r adiation hybrid map resolves a discrepancy between the two major bovine lin kage maps in the PAR of BTA X.