Evaluation of effects of 1,3-dinitrobenzene on sperm motility of hamster using computer assisted semen analysis (CASA)

Aim: To evaluate the effects of 1,3-dinitrobenznene (mDNB) on sperm motilit y of hamster and to correlate the results with the fertility. Methods: Adul t male hamsters were gavaged with one of the 3 dose regimes of mDNB (1.5 mg daily for 3 weeks, 1.5 mg one day a week for 1 weeks and 1.0 mg 3 days a w eek for 2 weeks). Computer assisted semen analysis (CASA) was used to analy se the sperm motility parameters, curvilinear velocity (VCL) and straight l ine velocity (VSL) of sperm in distal corpus epididymides and distal cauda epididymides. In vitro fertilisation was carried out only for 1.5 mg mDNB d aily group to determine the sperm fertilising capacity. Results: There was a significant reduction in sperm velocity parameters at weeks 3 and 4 after treatment, which was correlated with a decline in sperm fertility. Conclus ion: Sperm velocity parameters may be used to determine the effect of a tox ic insult on the sperm function.