T. Nara et al., Strand exchange reaction in vitro and DNA-dependent ATPase activity of recombinant LIM15/DMC1 and RAD51 proteins from Coprinus cinereus, BIOC BIOP R, 285(1), 2001, pp. 92-97
Citations number
21
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
We previously cloned recA-homolog genes from a basidiomycete, Coprinus cine
reus, and obtained the recombinant proteins (Nara et at, Mot Gen, Genet. 26
2, 781-789, 1999, see Ref, 1; Nara and Sakaguchi, Biochem, Biophys. Res. Co
mmun. 275, 97-102, 2000, see Ref, 2), The primary purpose of the present st
udy was to characterize the biochemical properties of the recombinant LIM15
/DMC1 (CoLIM15) and RAD51 (CoRAD51) proteins. We purified the recombinant p
roteins, and their molecular masses were 37 and 35 kDa, respectively. Both
enzymes showed DNA-dependent ATPase activity and ATP-dependent strand excha
nge reaction in vitro. CoRad51 was a five- to sixfold stronger DNA-dependen
t ATPase and showed greater dependency on single-stranded DNA than CoLim15,
In meiosis, both enzymes were highly accumulated in the meiotic tissue at
leptotene and zygotene stages at which the homologous chromosomes pair, but
disappeared just before the pachytene stage at which they recombine, From
these and the previously reported results, we discuss here the relationship
s between the enzymes and meiosis, (C) 2001 Academic Press.