Characterization of the cyanobacterial ycf37: mutation decreases the photosystem I content

Citation
A. Wilde et al., Characterization of the cyanobacterial ycf37: mutation decreases the photosystem I content, BIOCHEM J, 357, 2001, pp. 211-216
Citations number
24
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL JOURNAL
ISSN journal
02646021 → ACNP
Volume
357
Year of publication
2001
Part
1
Pages
211 - 216
Database
ISI
SICI code
0264-6021(20010701)357:<211:COTCYM>2.0.ZU;2-S
Abstract
We have constructed and analysed a cyanobacterial mutant that lacks the put ative homologue of ycf37, the chloroplast open reading frame 37, which is c onserved in different algae, but missing in the plastome of higher plants. In this report we show that Ycf37 of Synechocystis sp. PCC 6803 contains th ree tetratrico-peptide repeat (TPR) units resembling the structural organiz ation of Ycf3, a protein that has been suggested to function as a chaperone during photosystem (PS) I complex formation. We demonstrate a light-activa ted transcript accumulation of this gent. Inactivation of ycf37 leads to a lower PSI/PSII ratio and a higher phycocyanin/chlorophyll ratio in Synechoc ystis cells. The observed alterations in the ycf37 mutants and the structur al organization of the gene product suggest a functional role in PSI stabil ity or assembly.