A rapid, sensitive high performance liquid chromatographic method for the determination of meropenem in pharmaceutical dosage form, human serum and urine
Y. Ozkan et al., A rapid, sensitive high performance liquid chromatographic method for the determination of meropenem in pharmaceutical dosage form, human serum and urine, BIOMED CHRO, 15(4), 2001, pp. 263-266
A new, simple, precise and rapid high performance liquid chromatographic me
thod was developed for the determination of meropenem in human serum, urine
and pharmaceutical dosage forms. Chromatography was carried out on an LC18
column using a mixture of 15 mM KH2PO4:acetonitrile:methanol (84:12:4; v/v
/v), adjusted to pH 2.8 with H3PO4 The proposed method was conducted using
a reversed-phase technique, UV monitoring at 307.6 nm and cefepime as an in
ternal standard. The: retention times were 5.98 and 7.47 min for cefepime a
nd meropenem, respectively. The detector response was linear over the conce
ntration range of 50-10,000 ng/mL. The detection Limit of the procedure was
found to be 22 ng/mL. The detection limit for meropenem in human plasma wa
s 108.4 ng/mL and the corresponding value in human urine was 179.3 ng/mL. N
o interference from endogenous substances in human serum, urine and pharmac
eutical preparation was observed. The proposed method is sufficiently sensi
tive for determination of the concentrations of meropenem and may have clin
ical application for its monitoring in patients receiving the drug. Copyrig
ht (C) 2001 John Wiley & Sons, Ltd.