Biosensors for direct determination of organophosphate pesticides

Direct, selective, rapid and simple determination of organophosphate pestic ides has been achieved by integrating organophosphorus hydrolase with elect rochemical and opitical transducers. Organophosphorus hydrolase catalyzes t he hydrolysis of a wide range of organophosphate compounds, releasing an ac id and an alcohol that can be detected directly. This article reviews devel opment, characterization and applications of organophosphorus hydrolase-bas ed potentiometric, amperometric and optical biosensors. (C) 2001 Elsevier S cience B.V. All rights reserved.