Screening tests were carried out on the toxicity of freshwater puffers
Tetraodon leiurus complex and Tetraoaon suvatii collected from Udonth
ani province, north-eastern Thailand. Toxicity was highest in the live
r and varied according to the location and season of fish catch. Fish
which were reared in tap water for 3 months reduced the toxicity subst
antially. Partial purification was achieved by an ultrafiltration tech
nique. Toxin components were consequently identified by high-performan
ce liquid chromatography. It was found that toxins separated from the
eggs, liver, skin and muscle of these puffers were composed of saxitox
in, neosaxitoxin and decarbamoylsaxitoxin. (C) 1997 Elsevier Science L
td.