Robustness study for the determination of biogenic amines by HPLC

Citation
R. Romero et al., Robustness study for the determination of biogenic amines by HPLC, CHROMATOGR, 53(9-10), 2001, pp. 481-484
Citations number
17
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
CHROMATOGRAPHIA
ISSN journal
00095893 → ACNP
Volume
53
Issue
9-10
Year of publication
2001
Pages
481 - 484
Database
ISI
SICI code
0009-5893(200105)53:9-10<481:RSFTDO>2.0.ZU;2-Y
Abstract
A robustness test for the separation and LC determination of eight biogenic amines (histamine, tyramine, phenylethylamine, tryptamine, cadaverine, put rescine, spermidine and spermine) has been made in order to validate the me thod, which involves derivatization of the amines with dabsyl chloride, and analysis by reversed-phase liquid chromatography with gradient elution and spectrophotometric detection at 446 nm.