Comparision of high-resolution gas chromatography and high-performance liquid chromatography for simultaneous determination of lamotrigine and carbamazepine in plasma
Mec. Queiroz et al., Comparision of high-resolution gas chromatography and high-performance liquid chromatography for simultaneous determination of lamotrigine and carbamazepine in plasma, CHROMATOGR, 53(9-10), 2001, pp. 485-489
Rapid, simple and sensitive methods have been devised for simultaneous dete
rmination of lamotrigine (LTG) and carbamazepine (CBZ) in microsamples of h
uman plasma by high-performance liquid chromatography (HPLC) and high resol
ution gas chromatography (HRGC); both methods have been validated and compa
red. The HPLC method was more sensitive than HRGC for both drugs, LTG and C
BZ. The lowest quantitation limits (LOQ) of plasma LTG concentrations were
0.1 mug.mL(-1) (HPLC) and 0.5 mug.mL(-1) (HRGC); for CBZ they were 0.1 mug.
ml(-1) (HPLC) and 0.25 mug.mL(-1) (HRGC). Both methods, are sensitive enoug
h to work with microsamples at subtherapeutic, therapeutic and toxic concen
trations, being reliable and practical for routine therapeutic monitoring o
f LTG. The HPLC method proposed is suitable also for pharmacokinetic studie
s orchid patient, routine therapeutic-drug monitoring where there is a need
for sensitive methods and small sample sizes.