Comparative evaluation of three commercial software packages for analysis of DNA polymorphism patterns

Objective In the present study we have compared three commercial software p ackages, GelCompar, Molecular Analyst Fingerprinting, and BioImage, to dete rmine if the results generated by the programs were comparable and correlat ed adequately with visual interpretation of electrophoretic gels, in the an alysis of several well characterized incidents of infections. Methods Infections caused by Pseudomonas aeruginosa, Candida dubliniensis, C. albicans, and serotypes of Salmonella were characterized by restriction endonuclease analysis, macrorestriction analysis of genomic DNA with pulsed -field gel electrophoresis, and random amplified polymorphic DNA. The genot ypes were visually detected based on band presence or absence in the differ ent gels. The similarity values of DNA profiles were computed using Dice co efficient and were presented in dendrograms by upgma. The concordance or ag reement between the number of genotypes obtained and their clustering, usin g the computerized programs, was determined. Results In general, agreement in number of genotypes obtained visually and by using the commercial DNA analysis software was achieved, but discrepanci es were also denoted between the systems. The concordance between the visua l and the computerized analysis ranged from 72% to 100%. Conclusion In our experience, although the programs evaluated in the presen t study performed acceptably well, such programs may be used as an aid in t he analysis of complex banding patterns, and they do not provide an indispu tably correct analysis in genotype definition.