Phenotypic and genotypic markers of Staphylococcus epidermidis virulence

Objectives To analyze Staphylococcus epidermidis strains, previously tested for their virulence in a mouse model of subcutaneous infection, for variou s phenotypic trails (biofilm density, extracellular polysaccharide. slime-a ssociated antigen (SAA)) and for the presence of the ica gene cluster, to d etermine which of these phenotypic and genotypic methods best correlates wi th virulence in the mouse model. Methods The quantitative biofilm assay was performed on 10 strains of S. ep idermidis, comprising (1) RP62A (ATCC 35984), (2) the strongest: and weakes t biofilm producers in our collection, (3) a pair of phenotypic variants, a nd (4) a strain whose biofilm density was enhanced in iron-limited media. B iofilm density was measured after growth at 37 degreesC and at ambient temp erature, in trypticase soy broth (TSB) with and without glucose supplementa tion and using both chemical and heat fixation. Strains were assayed for SA A using a double immunodiffusion method. Extracellular polysaccharide was d etected by transmission electron microscopy (TEM). A 546-base-pair segment of the ica gene cluster was amplified by PCR. Results Biofilm formation in TSB, glucose-enriched TSB, extracellular polys accharide (observed by TEM), expression of SAA and presence of the ii a gen e predicted virulence of nine, nine, nine, eight and eight of 10 strains, r espectively. The phenotypic expression of biofilm and related properties wa s medium and temperature dependent. We encountered one ica-positive strain that failed to express biofilm in standard TSB at 37 degreesC, but was viru lent in a mouse model, and another strain that lacked ica, produced biofilm and was virulent in the model. Conclusions Mouse virulence in our model can be predicted by any of the phe notypic or genotypic methods examined for greater than or equal to 80% of s trains. Medium and incubation conditions affect the expression of phenotypi c markers by some strains. For the remaining strains, possible reasons For inconsistencies between the presence of the ica gene, phenotypic markers an d mouse virulence include (1) dependence of biofilm on genes other than ica , (2) sequence differences in ica, (3) dependence of biofilm expression in vivo on strain characteristics and media used to prepare inocula for in viv o studies.