Dr. Jung et al., Topographical and physicochemical modification of material surface to enable patterning of living cells, CR R BIOTEC, 21(2), 2001, pp. 111-154
Precise control of the architecture of multiple cells in culture and in viv
o via precise engineering of the material surface properties is described a
s cell patterning. Substrate patterning by control of the surface physicoch
emical and topographic features enables selective localization and phenotyp
ic and genotypic control of living cells. In culture, control over spatial
and temporal dynamics of cells and heterotypic interactions draws inspirati
on from in vivo embryogenesis and haptotaxis. Patterned arrays of single or
multiple cell types in culture serve as model systems for exploration of c
ell-cell and cell-matrix interactions. More recently, the patterned arrays
and assemblies of tissues have found practical applications in the fields o
f Biosensors and cell-based assays for Drug Discovery. Although the field o
f cell patterning has its origins early in this century, an improved unders
tanding of cell-substrate interactions and the use of microfabrication tech
niques borrowed from the microelectronics industry have enabled significant
recent progress. This review presents the important early discoveries and
emphasizes results of recent state-of-the-art cell patterning methods. The
review concludes by illustrating the growing impact of cell patterning in t
he areas of bioelectronic devices and cell-based assays for drug discovery.