Molecular cloning and characterization of a novel peptidylprolyl isomerase(cyclophilin)-like gene (PPIL3) from human fetal brain

During the large-scale sequencing analysis of a human fetal brain cDNA libr ary, we isolated two cDNA clones encoding two novel proteins, which show 52 % and 72 % identity to the cyclophilin isoform 10 of C. elegans, respectiv ely. Sequence analysis revealed these two cDNA clones are two different spl icing variants of a novel cyclophilin-like gene (PPIL3). The PPIL3 gene was identified on a completely sequenced BAC (GenBank accession AC005037) from chromosome 2q33 between STS markers stSG2762 (proximal) and SHGC-3074 (dis tal), oriented toward the telomere. The PPIL3 gene consisted of eight exons spanning more than 18 kb of genomic DNA. RT-PCR analysis indicated that PP IL3 was ubiquitously expressed in adult human tissues. Copyright (C) 2001 S . Karger AG, Basel.