Identification and isolation of a full-length clone of mouse GMFB (Gmfb), a putative intracellular kinase regulator, differentially expressed in telencephalon

We identified new transcribed sequences, using a differential display parad igm to select genes expressed in proliferating neuroblasts from mouse telen cephalon at 10 days of embryonic development. In this systematic search, we isolated a 361-bp partial 3' untranslated region (3' UTR) homologous to th e 3' UTR of the human gene encoding a putative intracellular kinase regulat or, glia maturation factor beta (GMFB). We cloned a full-length. 4,311-bp m ouse cDNA containing a 270-bp 5' UTR. a 3,615-bp 3' UTR, and an open readin g frame of 426 nucleotides encoding a putative 142 amino-acid protein, iden tical to human GMFB, with the exception of two amino acids. This 4.3-kb tra nscript is present in a variety of adult tissues and is developmentally reg ulated as shown by Northern blot analysis. Differential expression in telen cephalon was demonstrated by quantification of radioactive relative RT-PCR and confirmed by in situ hybridization. The isolation of this full-length c lone of mouse Gmfb should facilitate investigation of the intracellular mec hanisms involved in the develop ment of telencephalon. Copyright (C) 2001 S . Karger, AG, Basel.