Two distinct domains of Bicoid mediate its transcriptional downregulation by the Torso pathway

The transcriptional activity of the Bicoid morphogen is directly downregula ted by the Torso signal transduction cascade at the anterior pole of the Dr osophila embryo. This regulation does not involve the homeodomain or direct phosphorylation of Bicoid, We analyse the transcriptional regulation of Bi coid in response to the Torso pathway, using Bicoid variants and fusion pro teins between the Bicoid domains and the GaI4 DNA-binding domain, We show t hat Bicoid possesses three autonomous activation domains. Two of these doma ins, the serine/threonine-rich and the acidic domains, are downregulated by Torso, whereas the third activation domain, which is rich in glutamine, is not. The alanine-rich domain, previously described as an activation domain in vitro, has a repressive activity that is independent of Torso. Thus, Bi coid downregulation by Torso results from a competition between the glutami ne-rich domain that is insensitive to Torso and the serine/threonine-rich a nd acidic activation domains downregulated by Torso. The alanine-rich domai n contributes to this process indirectly by reducing the global activity of the protein and in particular the activity of the glutamine-rich domain th at might otherwise prevent downregulation by Torso.