A monoclonal antibody against the immunodominant epitope of the ribosomal P2 beta protein of Trypanosoma cruzi interacts with the human beta 1-adrenergic receptor
E. Mahler et al., A monoclonal antibody against the immunodominant epitope of the ribosomal P2 beta protein of Trypanosoma cruzi interacts with the human beta 1-adrenergic receptor, EUR J IMMUN, 31(7), 2001, pp. 2210-2216
Monoclonal antibodies were raised against a recombinant ribosomal P2 beta p
rotein of Trypanosoma cruzi, One of these reacted with the C terminus of th
is protein (peptide R13, EEEDDDMGFGLFD) and epitope mapping confirmed that
this epitope was the same as the one defined by the serum of immunized mice
, and similar to the previously described chronic Chagas' heart disease (cC
hHD) anti-P epitope. Western blotting showed that the monoclonal antibody r
ecognized the parasite ribosomal P proteins, as well as the human ribosomal
P proteins. Electron microscopy showed that it stained different structure
s in parasite and human cells. interestingly, surface plasmon: resonance me
asurements indicated that the affinity for the parasite ribosomal P protein
epitope (R63) was five times higher than for its human counterpart (peptid
e H13, EESDDDMGFGLFD). Since the human epitope contained an acidic region (
EESDDJ similar to the AESDE peptide recognized by cChHD patients in the sec
ond extra-cellular loop of the human beta1-adrenergic -adrenergic receptor,
the biological activity of the antibody was assessed on neonatal: rat card
iomyocytes in culture. The monoclonal antibody had an agonist-like effect.
These results, together with the fact that the monoclonal reacted in Wester
n blots with the different isoforms of the heart beta1-adrenergic receptor,
confirm the possible pathogenic role of antibodies against the parasite ri
bosomal P protein based on their cross-reaction with the human beta1-adrene
rgic receptor.