Ed. Hu et al., Molecular cloning and characterization of profilin-3: A novel cytoskeleton-associated gene expressed in rat kidney and testes, EXP NEPHROL, 9(4), 2001, pp. 265-274
Profilin is a small actin-binding protein that is involved in diverse funct
ions such as maintaining cell structure integrity, cell mobility, tumor cel
l metastasis, as well as growth factor signal transduction. In this paper,
we describe the molecular cloning and characterization of a novel form of p
rofilin, termed profilin-3, from rat kidney using a PCR-based procedure for
isolating tissue-specific genes. The profilin-3 cDNA encoded 137 amino aci
ds, and it shared extensive homology to profilin-1 and profilin-2 from mice
and humans. More strikingly, the expression of profilin-3 was highly selec
tive, and its mRNA was only found in the kidney and to a much lesser extent
in the testis. The size of the mRNA for profilin-3 in the testis was 1.2 k
b, while in kidney it was approximately 4.4-5 kb, suggesting the presence o
f tissue-specific transcription from different promoters. In addition, we a
lso found that the expression of profilin-3 mRNA was significantly elevated
in two types of renal diseases: diabetic nephropathy (db/db mice) and poly
cystic kidney diseases (cpk mice). Similar to profilin-l and profilin-2, pr
ofilin-3 was localized in the cytoplasmic domain. Furthermore, it was capab
le of interacting with actin and poly-L-proline in an in vitro assay as wel
l as in transfected cells. These results strongly suggest that, contrary to
the ubiguitous presence of profilin-1 and profilin-2, there is a tissue-sp
ecific form of these cytoskeleton-regulatory proteins and that the kidney/t
estes-specific profilin-3 may play a unique role in renal and/or reproducti
ve functions. Copyright (C) 2001 S. Karger AG, Basel.