Jg. Bundy et al., An NMR-based metabonomic approach to the investigation of coelomic fluid biochemistry in earthworms under toxic stress, FEBS LETTER, 500(1-2), 2001, pp. 31-35
The endogenous metabolites of the coelomic fluid of the earthworm Eisenia v
eneta were characterised using high-resolution one-dimensional and two-dime
nsional H-1 nuclear magnetic resonance spectroscopy, Signals from common or
ganic acids, such as acetate, fumarate, malonate, malate, formate, and succ
inate, mere identified together with adenosine and nicotinamide mononucleot
ide, The potential use of this information as a baseline dataset for future
toxicological or physiological studies was demonstrated by a metabonomic a
nalysis: a series of earthworms were dosed,vith the model compound 3-fluoro
-4-nitrophenol, and toxic effects followed by multivariate analysis of the
spectral data of the coelomic fluid. Relative concentrations of acetate and
malonate were decreased in the dosed worms compared to the controls, (C) 2
001 Published by Elsevier Science B.V. on behalf of the Federation of Europ
ean Biochemical Societies.