A high-resolution consensus linkage map of the rat, integrating radiation hybrid and genetic maps

We have constructed a high-resolution consensus genetic map of the rat in a single large intercross, which integrates 747 framework markers and 687 po sitions of our whole-genome radiation hybrid (RH) map of the rat. We select ed 136 new gene markers from the GenBank database and assigned them either genetically or physically to rat chromosomes to evaluate the accuracy of th e integrated linkage-RH maps in the localization of new markers and to enri ch existing comparative mapping data. These markers and 631 D-Got- markers, which are physically mapped but still uncharacterized for evidence of poly morphism, were tested for allele variations in a panel of 16 rat strains co mmonly used in genetic studies. The consensus linkage map constructed in th e GK x BN cross now comprises 1620 markers of various origins, defining 840 resolved genetic positions with an average spacing of 2.2 cM between adjac ent loci, and includes 407 gene markers. This whole-genome genetic map will contribute to the advancement of genetic studies in the rat by incorporati ng gene/EST maps, physical mapping information, and sequence data generated in rat and other mammalian species into genetic intervals harboring diseas e susceptibility loci identified in rat models of human genetic disorders.