Disruption of imprinted expression of U2afbp-rs/U2af1-rs1 gene in mouse parthenogenetic fetuses

Citation
Y. Sotomaru et al., Disruption of imprinted expression of U2afbp-rs/U2af1-rs1 gene in mouse parthenogenetic fetuses, J BIOL CHEM, 276(28), 2001, pp. 26694-26698
Citations number
39
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
00219258 → ACNP
Volume
276
Issue
28
Year of publication
2001
Pages
26694 - 26698
Database
ISI
SICI code
0021-9258(20010713)276:28<26694:DOIEOU>2.0.ZU;2-G
Abstract
The present study shows that the U2afbp-rs gene, a paternally expressed imp rinted gene, is activated and expressed in a biallelic manner from maternal alleles in parthenogenetic mouse fetuses on day 9.5 of gestation. The mean expression was detected to be 88% (31-134%) of that in control biparental fetuses, using real-time quantitative reverse transcription and polymerase chain reaction. This disrupted expression of the gene was associated with c hanges in the chromatin structure but not with the methylation pattern in t he regulation region. The present results show that parental specific expre ssion of imprinted genes is not always maintained in uniparental embryos. T his suggests that both parental genomes are necessary to establish parental specific expression of the U2afbp-rs gene.