FLASH coordinates NF-kappa B activity via TRAF2

FLASH is a protein recently shown to interact with the death effector domai n of caspase-8 and is likely to be a component of the death-inducing signal ing complex in receptor-mediated apoptosis, Here we show that antisense oli gonucleotide-induced inhibition of FLASH expression abolished TNF-ru-induce d activation of NF-KB in HEK293 cells, as determined by luciferase reporter gene expression driven by a NF-KB responsive promoter. Conversely, overexp ression of FLASH dose-dependently activated NF-KB, an effect suppressed by dominant negative mutants of TRAF2, NIK, and IKK alpha, and partially by th ose of TRAF5 and TRAF6, TRAF2 was co-immunoprecipitated with FLASH from the cell extracts of HEK293 cells or HeLa cells stably expressing exogenous FL ASH (HeLa/HA-FLASH), Furthermore, serial deletion mapping demonstrated that a domain spanning the residues 856-1191 of FLASH activated NF-KB as effici ently as the full-length and could directly bind to TRAF2 in vitro and in t he transfected cells. Taken together, these results suggest that FLASH coor dinates downstream NF-KB activity via a TRAF2-dependent pathway in the TNF- alpha signaling.