G. Schmidmaier et al., Biodegradable poly(D,L-lactide) coating of implants for continuous releaseof growth factors, J BIOMED MR, 58(4), 2001, pp. 449-455
Local application of growth factors like insulin like growth factor-I (IGF-
I) and transforming growth factor-beta 1 (TGF-beta1) from a biodegradable t
hin layer of poly(D,L-lactide) (PDLLA) coated implants could stimulate frac
ture healing. A new "cold coating technique" for metallic implants was esta
blished to produce a biodegradable coating with a high mechanical stability
that provides a continuous release of incorporated growth factors. The pro
perties of this bioactive coating were investigated in vitro and in vivo. S
canning electron microscope analysis revealed a coating thickness of in ave
rage 14.8 mum on titanium and 10.7 mum on steel wires. Intramedullary impla
ntation and extraction experiments depicted a loss of PDLLA coating from ti
tanium and steel implants of less than 5%, After explantation of the implan
ts, the coating displayed a complete and regular layer without any defects
of PDLLA uncovering the metallic surface. Smear tests demonstrate that the
coating can be performed under sterile conditions. The PDLLA depicted a red
uction of about 8% within 6 weeks in vitro and in vivo. The growth factors
were incorporated in a stable form and demonstrated a loss of stability of
less than 3% within 42 days and less than 5% within one year. In an elution
experiment, 54% IGF-I and 48% TGF-beta1 were released within the first 48
h, After 42 days, 76% of IGF-I and 71% of TGF-beta1 were detected in the el
ution fluid by ELISA, Comparable results were obtained in the in vive exper
iments after 42 days. (C) 2001 John Wiley & Sons, Inc.