Systematic analysis of acid, neutral and basic drugs in horse plasma by combination of solid-phase extraction, non-aqueous partitioning and gas chromatography-mass spectrometry

A sample preparation method for mass chromatographic detection of doping dr ugs from horst plasma is described. Bond Elut Certify (1 g/6 ml) is used fo r the extraction of 4 ml of horst: plasma. Fractionation is performed with 6 ml of CHCl3-Me2CO (8:1) and 5 ml of 1% TEA-MeOH according to its property . Simple and effective clean-up based on non-aqueous partitioning is adopte d to remove co-eluted contaminants in both acid and basic fractions. Two ki nds of 1-(N,N-diisopropylamino)-n-alkanes are co-injected with the sample i nto the GC-MS system for the calculation of the retention index. Total reco veries of 107 drugs are examined. Some data of post administration plasma a re presented. This procedure achieves sufficient recoveries and clean extra cts for GC-MS analysis. The method is able to detect ng/ml drug levels in h orse plasma. (C) 2001 Elsevier Science B.V. All rights reserved.