Determination of amlodipine in human plasma by high-performance liquid chromatography with fluorescence detection

A sensitive and specific HPLC method has been developed for the assay of am lodipine in human plasma. The assay involves derivatization with 4-chloro-7 -nitrobenzofurazan (NBD-Cl), solid-phase extraction on a silica column and isocratic reversed-phase chromatography with fluorescence detection. Nortri ptyline hydrochloride was used as an internal standard. The assay was linea r over the concentration range of 0.25-18.00 ng/ml. Both of the within-day and day-to-day reproducibility and accuracy were less than 11.80% and 12.00 %, respectively. The plasma profile following a single administration of 10 mg amlodipine to a healthy volunteer was presented. (C) 2001 Elsevier Scie nce B.V. All rights reserved.