Enhancement of an insufficient dye-formation in the ninhydrin reaction by a suitable post treatment process

A study of the reaction between ninhydrin and alanine has been carried out taking into account that, adhered on the surface of a dry porous medium suc h as paper, a quasi-heterogeneous reaction has to take place. Instead of am ino acids released from human sweat glands, aqueous solutions of alanine we re taken to deliver a given amount of amino acid to the sample. The dye den sity, obtained after using a standard development process, could noticeably be increased by setting a drop of water on the dye dot, thus indicating th at not all the alanine had been used for dye formation during the usually a pplied process. The incomplete reaction can be explained by the problem of bringing the reactants into contact with each other when both are in the so lid phase in the porous surrounding. The temporary presence of water allows a reorientation of the insoluble reactants. With fingerprints an increase in both the rate of development and the final dye density could be obtained when the sample was post treated after the developing process with the bla nk solvent, thus also the background coloration could be decreased. The ide as presented in this paper may form the basis for a modification of develop ing processes with ninhydrin in order to increase the proportion of amino a cids present tin the sample) used in dye formation without data loss.