PKC gamma contributes to a subset of the NMDA-dependent spinal circuits that underlie injury-induced persistent pain

In previous studies we provided evidence that the gamma isoform of protein kinase C (PKC gamma) is an important contributor to the increased pain sens itivity that occurs after injury. Here we combined electrophysiological and behavioral approaches in wild-type and PKC gamma -null mice to compare the hyperexcitability of wide dynamic range neurons in lamina V of the spinal cord dorsal horn with the behavioral hyperexcitability produced by the same injury [application of a C-fiber irritant, mustard oil (MO), to the hindpa w]. Wild-type and null mice did not differ in their response to mechanical or thermal stimuli before tissue injury, and the magnitude of the response to the MO stimuli was comparable. in wild-type mice, MO produced a dramatic and progressive enhancement of the response of lamina V neurons to innocuo us mechanical and thermal stimuli. The time course of the neuronal hyperexc itability paralleled the time course of the MO-induced behavioral allodynia (nocifensive behavior in response to a previously innocuous mechanical sti mulus). Neuronal hyperexcitability was also manifest in the PKC gamma -null mice, but it lasted <30 min. By contrast, the behavioral allodynia produce d by MO in the PKC<gamma>-null mice, although reduced to approximately half that of the wild-type mice, persisted long after the lamina V hyperexcitab ility had subsided. Because the MO-induced behavioral allodynia was complet ely blocked by an NMDA receptor antagonist, we conclude that PKC gamma medi ates the transition from short- to long-term hyperexcitability of lamina V nociresponsive neurons but that the persistence of injury-induced pain must involve activity within multiple NMDA-dependent spinal cord circuits.