Configuration and racemization determination of cysteine residues in peptides by chiral derivatization and HPLC: Application to oxytocin peptides

Authors
Szabo, S Szokan, G Khlafulla, AM Almas, M Kiss, C Rill, A Schon, I
Citation
S. Szabo et al., Configuration and racemization determination of cysteine residues in peptides by chiral derivatization and HPLC: Application to oxytocin peptides, J PEPT SCI, 7(6), 2001, pp. 316-322
Citations number
27
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF PEPTIDE SCIENCE
ISSN journal
10752617 → ACNP
Volume
7
Issue
6
Year of publication
2001
Pages
316 - 322
Database
ISI
SICI code
1075-2617(200106)7:6<316:CARDOC>2.0.ZU;2-C
Abstract
An improved RP-HPLC method was developed for the determination of the confi guration and stereochemical purity of cysteine residues in peptides. The me thod consists of oxidation of cysteine and cystine residues to cysteic acid , followed by hydrolysis and pre-column chiral derivatization with Val-Marf ey's reagent. Copyright (C) 2001 European Peptide Society and John Wiley & Sons, Ltd.