Surface properties of polystyrene nanoparticles coated with dextrans and dextran-PEO copolymers. Effect of polymer architecture on protein adsorption

Hydrophobically substituted dextran (dextran phenoxy, DexP) and dextran phe noxy-poly( ethylene oxide) copolymers (DexP-PEO) have been used to modify t he surface of polystyrene latex particles. To avoid polymer desorption in t he presence of hydrophobic species such as proteins, the adsorbed layer was stabilized by chemical cross-linking and then characterized in terms of ad sorbed amount, thickness, and stability. The interfacial concentration in a nchoring phenoxy groups and the PEO grafting density were both varied, and their effects on nonspecific bovine serum albumin (BSA) adsorption were exa mined. It was found that the most important parameter in preventing BSA ads orption is the number of interactions between the adsorbed dextran and the surface, even in the presence of DexP-PEO layers with high grafting ratios of PEO chains. We also examined the ability of dextran layers to bind speci fically concanavalin A (Con A) as the Con A molecule exhibits a good specif ic affinity for glucose-containing carbohydrates. Flocculation of DexP-modi fied particles by Con A was observed in the course of the experiments. All of these results are discussed in relation to the importance of polymer arc hitecture and surface-protein interactions in protein rejection by dextran and dextran-PEO coatings.