L. Soltes et al., Radical degradation of high molecular weight hyaluronan: Inhibition of thereaction by ibuprofen enantiomers, METH FIND E, 23(2), 2001, pp. 65-71
Citations number
36
Categorie Soggetti
Pharmacology & Toxicology
Journal title
METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY
The antioxidative and/or free-radical-scavenging activities of R-(-)- and S
-(+)-ibuprofen enantiomers, as well as of the drug racemate, were studied i
n vitro on measuring the kinetics of (uninhibited or drug-inhibited) degrad
ation of high molecular weight hyaluronan by hydroxyl radicals. The continu
al flux of OH radicals at aerobic conditions was maintained by the H2O2 +Ca
2+ system. The kinetics of hyaluronan degradation was monitored indirectly
by capillary viscometry. under experimental conditions, with no drug additi
on, the relative viscosity ([eta](rel)) decreased continuously, reaching 13
% of the initial [eta](rel.)value in 4 h. Each drug tested exhibited a dose
-dependent protective effect against hyaluronan degradation, however R-(-)-
ibuprofen demonstrated a slightly greater activity than the drug S-(+)-enan
tiomer. (C) 2001 Prous Science. All rights reserved.