Dj. Conway et al., Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci, MOL BIOCH P, 115(2), 2001, pp. 145-156
Comparing patterns of genetic variation at multiple loci in the genome of a
species can potentially identify loci which are under selection. The large
number of polymorphic microsatellites in the malaria parasite P. Plasmodiu
m falciparum are available markers to screen for selectively important loci
. The Pfs48/45 gene on Chromosome 13 encodes an antigenic protein located o
n the surface of parasite gametes, which is a candidate for a transmission
blocking vaccine. Here, genotypic data from 255 P. falciparum isolates are
presented, which show that alleles and haplotypes of five single nucleotide
polymorphisms (SNPs) in the Pfs48/45 gene are exceptionally skewed in freq
uency among different P. falciparum populations. compared with alleles at 1
1 microsatellite loci sampled widely from the parasite genome. Fixation ind
ices measuring inter-population variance in allele frequencies (F-ST) were
in the order of four to seven times higher for Pfs48/45 than for the micros
atellites, whether considered (i) among populations within Africa, or (ii)
among different continents. Differing mutational processes at microsatellit
e and SNP loci could generally affect the population structure at these dif
ferent types of loci, to an unknown extent which deserves further investiga
tion. The highly contrasting population structure may also suggest divergen
t selection on the amino acid sequence of Pfs48/45 in different populations
, which plausibly indicates a role for the protein in determining gamete re
cognition and compatibility. (C) 2001 Elsevier Science B.V. All rights rese
rved.