Orexin (hypocretin) gene expression in rat ependymal cells

Authors
Kummer, M Neidert, SJ Johren, O Dominiak, P
Citation
M. Kummer et al., Orexin (hypocretin) gene expression in rat ependymal cells, NEUROREPORT, 12(10), 2001, pp. 2117-2120
Citations number
24
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROREPORT
ISSN journal
09594965 → ACNP
Volume
12
Issue
10
Year of publication
2001
Pages
2117 - 2120
Database
ISI
SICI code
0959-4965(20010720)12:10<2117:O(GEIR>2.0.ZU;2-R
Abstract
The expression of prepro-orexin (PPO) mRNA in the rat brain was investigate d by in situ hybridization histochemistry. In the lateral and posterior hyp othalamic areas, which are considered to produce exclusively PPO mRNA, we f ound high levels of PPO mRNA expressions. We also localized PPO mRNA hybrid ization signals at lower levels around the lateral ventricles, the third an d fourth ventricle. Cellular analysis by emulsion autoradiography revealed the expression of PPO mRNA in the ependymal cell layer. Our results demonst rate that beside the lateral and posterior hypothalamus PPO mRNA is express ed in ependymal cells. NeuroReport 12:2117-2120 (C) 2001 Lippincott William s & Wilkins.