Isolation of a cDNA encoding an IgG immunoreactive antigen (zinc finger protein) of Strongyloides stercoralis

A full length cDNA encoding an IgG immunoreactive antigen of Strongyloides stercoralis is described. A clone containing 1,328 bp insert was selected f ollowing screening of S, stercoralis cDNA library with an igG fraction obta ined from a pool of 78 S, stercoralis positive human sera samples. The nucl eotide sequence of the 1,328 bp insert was found to be 70.5 % A/T, reflecti ng a characteristic A/T codon bias of S, stercoralis. The nucleotide sequen ce of this insert identified a cDNA coding for a zinc finger protein. The c onceptually translated amino acid sequence of the open reading frame for th e igG immunoreactive antigen of S, stercoralis encodes a 211 amino acid res idue protein with on apparent molecular weight of 22.8 kDa and a predicted isoelectric point of 8.71. The diagnostic potential of this IgG immunoreact ive antigen of S, stercoralis is also discussed.