Development of systems for targeting the regional lymph nodes for diagnostic imaging: In vivo behaviour of colloidal PEG-coated magnetite nanospheresin the rat following interstitial administration

Purpose. Nanoparticles can be utilised for targeting drugs to the regional lymph nodes or as diagnostic agents. The surface modification of magnetite nanospheres with poly(ethylene glycol) (PEG) has been assessed by in vitro characterisation and in vivo studies following subcutaneous administration to the rat. Methods. Magnetite nanospheres were prepared with a grafted PEG layer using various PEG lengths from 350 to 1000 Da. Thermogravimetric analysis was ut ilised to measure the adsorbed amount of PEG. Colloid stability was confirm ed by measurement of the particle size and electrophoretic mobility. The ki netics of injection site drainage and lymph node retention were determined 2 hours after subcutaneous administration, for nanospheres coated with PEG lengths of 350. 550, 750, and 1000 Da, For the 750 PEG coated nanospheres, the kinetics of distribution was determined over a 18-hour time course. Results. The distribution of the nanospheres was modified and the lymph nod e localisation enhanced by altering the surface coverage of PEG on the magn etic surface. Conclusions. PEG-coated magnetite nanospherrs with different surface charac teristics can be utilised to target a diagnostic agent to regional lymph no des.