We investigated the effects of dietary alpha -linolenic acid (LNA; 18:3n-3)
of laying hens on the fatty acid composition of liver microsomes and activ
ity of Delta -6 desaturase in hatched chicks. Laying hens were fed wheat-so
ybean meal-based diets with (Flax) or without ground flax (control). At Day
21 of feeding, fertile eggs were collected and incubated. On the day of ha
tching, chicks (n = 6) were killed, and liver microsomes were isolated and
assayed for Delta -6 desaturase enzyme activity. The total n-3 fatty acids
in the Flax eggs was 13.0% compared with 2.3% (P < 0.001) in the control gr
oup. LNA was the major n-3 fatty acid in the Flax eggs and constituted 10%.
The long-chain n-3 polyunsaturated fatty acids (PUFA) (20:5n-3, 22:5n-3 an
d 22:6n-3) constituted 3.1% in Flax eggs compared with 1.5% in control eggs
. Docosahexaenoic acid (22:6n-3) composed 2.1 and 1.2% in Flax and control
eggs (P < 0.05), respectively. The liver microsomes of Flax chicks incorpor
ated higher 18:3n-3, 20:5n-3, and 22:5n-3 (P < 0.05) with a concomitant red
uction in 20:4n-6, 22:4n-6, and 22:5n-6 compared with control chicks (P < 0
.05). The Delta -6 desaturase activities in Flax and control groups were 49
.4 and 82.8 pmol/min per mg of protein, respectively (P < 0.05). These resu
lts demonstrate the role of maternal and yolk n-3 fatty acids in modulating
the activity of rate-limiting enzymes for PUFA synthesis in hatched chicks
.