Protection from superoxide damage associated with an increased level of the YggX protein in Salmonella enterica

Authors
Gralnick, J Downs, D
Citation
J. Gralnick et D. Downs, Protection from superoxide damage associated with an increased level of the YggX protein in Salmonella enterica, P NAS US, 98(14), 2001, pp. 8030-8035
Citations number
59
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
00278424 → ACNP
Volume
98
Issue
14
Year of publication
2001
Pages
8030 - 8035
Database
ISI
SICI code
0027-8424(20010703)98:14<8030:PFSDAW>2.0.ZU;2-D
Abstract
The deleterious effect of superoxide radicals on cell growth and survival i s predominately caused by rapid oxidation of labile [Fe-S] clusters in prot eins. Oxidation of these clusters releases Fe(II) ions, which participate i n Fenton chemistry that damages DNA. Here it is shown that elevated levels of the YggX protein increase the resistance of Salmonella enterica to super oxide stress, reverse enzymatic defects attributed to oxidized [Fe-S] clust ers, and decrease the spontaneous mutation frequency. The data are consiste nt with a model in which YggX protects protein [Fe-S] clusters from oxidati on.