The response of muscle interstitial prostaglandin E-2 (PGE(2)), prostacyclin I-2 (PGI(2)) and thromboxane A(2) (TXA(2)) levels during incremental dynamic exercise in humans determined by in vivo microdialysis
M. Karamouzis et al., The response of muscle interstitial prostaglandin E-2 (PGE(2)), prostacyclin I-2 (PGI(2)) and thromboxane A(2) (TXA(2)) levels during incremental dynamic exercise in humans determined by in vivo microdialysis, PROS LEUK E, 64(4-5), 2001, pp. 259-263
Citations number
21
Categorie Soggetti
Cell & Developmental Biology
Journal title
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS
The microdialysis in vivo technique allows the isolation, purification and
quantitative determination of bioactive molecules with low molecular weight
( <20.000 Da) from interstitial fluid (IF) of the muscles. PGE(2) and PGI(
2) are vasodilator local hormones, while the TXA(2) is a vasoconstrictor. P
GI(2) and TXA(2) are unstable and convert to stable products 6-keto-PGF(1a)
and TXB2, respectively. The purpose of this study was to evaluate the resp
onse of PGE(2), PGI(2) and TXA(2) in the IF of human muscle (vastus lateral
is) during dynamic exercise with a cycle ergometer. In this study two micro
dialysis probes were inserted with CMA-60 microdialysis catheters into the
vastus lateralis muscle of the right leg of eight healthy volunteers aged 2
4.1<plus/minus>2.1 years, height 177.5 +/-1.5 cm and body weight 78.1 +/-2.
4 kg. After insertion the microdialysis probes perfused at a rate of 3.0 mu
l/min with Ringer acetate solution. The dialysate fluid was collected a) du
ring the 30' rest period, b) during the 30' exercise period at 100 watts, c
) during the 30' exercise period at 150 watts and d) during the 30' rest pe
riod after exercise. Our measurements (by the RIA method) showed that the l
evels of PGE(2) and 6-keto-PGF(1a) in the I.F. of the vastus lateralis musc
le increased significantly, while there was a significant decrease in TXB2
during exercise. The changes in the above biomolecules were increased propo
rtionately with the strain of the subject's muscle. Conclusion: Dynamic exe
rcise of the muscles produces a local increase of the vasodilators PGE(2) a
nd PGI(2) while the vasoconstrictor TXA(2) is reduced in the IF of the musc
les. This is further evidence that exercise induces propitious biochemical
changes. Furthermore, the muscle production of arachidonic acid metabolites
during exercise depends on the intensity of the exercise. (C) 2001 Harcour
t Publishers Ltd.