C. Draveling et al., SwellGel: An affinity chromatography technology for high-capacity and high-throughput purification of recombinant-tagged proteins, PROT EX PUR, 22(2), 2001, pp. 359-366
The revolution in genomics and proteomics is having a profound impact on dr
ug discovery. Today's protein scientist demands a faster, easier, more reli
able way to purify proteins. A high capacity, high-throughput new technolog
y has been developed in Perbio Sciences for affinity protein purification.
This technology utilizes selected chromatography media that are dehydrated
to form uniform aggregates. The SwellGel aggregates will instantly rehydrat
e upon addition of the protein sample, allowing purification and direct per
formance of multiple assays in a variety of formats. SwellGel technology ha
s greater stability and is easier to handle than standard wet chromatograph
y resins. The microplate format of this technology provides high-capacity,
high-throughput features, recovering milligram quantities of protein suitab
le for high-throughput screening or biophysical/structural studies. Data wi
ll be presented applying SwellGel technology to recombinant 6x His-tagged p
rotein and glutathione-S-transferase (GST) fusion protein purification. (C)
2001 Academic Press.