Basal forebrain microinjections of L-3,3 ',5-triiodothyronine modify sleepin hypothyroid rats

Male rats were injected bilaterally with various doses of L-3,3',5-triiodot hyronine (T-3)) into basal forebrain areas. The electroencephalogram (EEG), electromyogram (EMG) and brain temperature (Tbr) were then measured in 8-h studies. In the medial preoptic area (MPA), a 4 mug dose of T-3 caused sig nificant elevations in REM sleep as compared to control injections. In the median preoptic nucleus (MnPO), both 2 and 4 mug doses of T-3 significantly inhibited non-REM sleep. Injections to the diagonal band of Broca did not alter EEG-defined sleep. Influences on Tbr were not significant for any of the treatments. Since these effects of T-3 were demonstrated after acute in jections, the data are consistent with possible non-genomic actions of thyr oid hormones in adult brain.