ROLE OF NATRIURETIC PEPTIDE RECEPTOR-TYPE-C IN DAHL SALT-SENSITIVE HYPERTENSIVE RATS

The natriuretic peptide system is suggested to be involved in the path ogenesis of salt-sensitive hypertension; a recent report indicated tha t disruption of the atrial natriuretic peptide precursor gene caused s alt-sensitive hypertension. However, natriuretic peptide receptor (NPR )-A knockout mice did not show enhanced salt sensitivity of blood pres sure. The aim of the present study was to investigate the role of NPR- C, the other receptor for atrial natriuretic peptide, in increased sal t sensitivity of blood pressure. Dahl salt-sensitive (DS) and salt-res istant (DR) rats were placed on a 0.3% or 8% NaCl diet for 4 weeks. Bl ood pressure was elevated by salt loading only in DS rats. RNase prote ction assay demonstrated that NPR-C transcript level in the kidney was reduced by chronic salt loading in both DR and DS rats, whereas expre ssion of NPR-A and NPR-B was not altered. The reduction of NPR-C mRNA in response to salt loading was enhanced in DS compared with DR rats. In situ hybridization indicated that the salt-induced NPR-C change was attributed mainly to suppressed expression of NPR-C in the podocytes. NPR-C gene expression was regulated by salt loading in a tissue-speci fic manner; the marked decrease in NPR-C mRNA by salt loading was seen only in the kidney. These data suggest that the exaggerated salt-indu ced reduction of NPR-C in the kidney of DS rats may play an important role in the pathogenesis of salt hypertension in this animal, possibly related to impaired renal sodium excretion.