TRANS-UTERINE TRACER INJECTIONS IN MAMMALIAN FETUSES

Citation
Eajf. Lakke et al., TRANS-UTERINE TRACER INJECTIONS IN MAMMALIAN FETUSES, Biological rhythm research, 28(3), 1997, pp. 306-318
Citations number
34
Categorie Soggetti
Biology,Physiology
Journal title
ISSN journal
09291016
Volume
28
Issue
3
Year of publication
1997
Pages
306 - 318
Database
ISI
SICI code
0929-1016(1997)28:3<306:TTIIMF>2.0.ZU;2-S
Abstract
Intra-uterine manipulation of mammalian foetuses for experimental purp oses was first described at the beginning of this century (Wolff, 1919 ). Though numerous publications have appeared since which bear witness to the feasibility of intra-uterine manipulation, its application has remained rather restricted. In this paper we describe a technique for the trans-uterine injection of neuronal tracers into rat foetuses. Ut erine wall and foetal membranes are pierced only with a micro pipette, and are thus left virtually intact, preventing loss of amniotic fluid . Surgical mortality is 24% overall, but the experimental success rate is much lower (23%). Even so these results are comparable to more com plicated procedures, because the technique is simple (i.e. requires no micro surgical skill), and because up to 6 foetuses can be injected p er dam. Technical problems. such as foetal anaesthesia and the detecti on of false-negative results due to imponderable factors are discussed .