Comparison of two platelet additive solutions

The use of an additive solution for substitution of plasma for storage of l eukodepleted platelet concentrates can have many advantages. In this study, a comparison was made between two platelet additive solutions: one contain ing citrate and acetate (PAS-LI), the other also supplemented with addition al salts such as magnesium, and with gluconate (Composol-PS). Donor-depende nt differences were avoided by applying a paired experimental design (n = 1 0). The platelet concentrates were prepared by pooling five buffy coats and the additive solution, and prestorage filtration was utilized to remove le ucocytes to well below 1 x 10(6). Storage of platelet concentrates up to 9 days after blood collection revealed that platelet concentrates in Composol -PS maintained an almost constant pH of on average 6.93 from day 2 through day 7, and at 6.90 at day 9. This was in contrast to PAS-II, which showed a gradually decreasing pH from on average 6.97 at day 1 to 6.86 at day 9. In all units stored in both solutions the swirling effect was present during 9 days of storage. In conclusion, both additive solutions allow storage of platelets, derived from pooled buffy coats, for up to 9 days after collecti on of the whole blood, with maintenance of good quality in vitro. Composol- PS has a slightly better buffering capacity, reflected as a more constant p H throughout the storage period.