A truncated HCV core protein elicits a potent immune response with a strong participation of cellular immunity components in mice

The immunogenicity of a truncated HCV core protein (Co.120) was studied in BALB/c and C57BL/6 mice, given three intramuscular injections of antigen, a djuvanted with either aluminum hydroxide or Freund's adjuvant. A rapid anti body response was noted after the first dose, with both strains of mice eve ntually exhibiting comparable levels of anti-core IgG (titers > 1:100000), with a mixed IgG1/IgG2a subclass response. Spleen cells from Co.120-immuniz ed mice gave a significant specific proliferative response. IFN-gamma gene expression was also detected after an ex-vivo specific stimulation of splee n cells in all immunized mice. This response was independent of dose, H-2 g enetic background or type of adjuvant. The results indicated that immunizat ion with the Co.120 protein elicits a potent anti-HCV humoral and cellular immune response. (C) 2001 Elsevier Science Ltd. All rights reserved.