Jc. Alvarez-obregon et al., A truncated HCV core protein elicits a potent immune response with a strong participation of cellular immunity components in mice, VACCINE, 19(28-29), 2001, pp. 3940-3946
The immunogenicity of a truncated HCV core protein (Co.120) was studied in
BALB/c and C57BL/6 mice, given three intramuscular injections of antigen, a
djuvanted with either aluminum hydroxide or Freund's adjuvant. A rapid anti
body response was noted after the first dose, with both strains of mice eve
ntually exhibiting comparable levels of anti-core IgG (titers > 1:100000),
with a mixed IgG1/IgG2a subclass response. Spleen cells from Co.120-immuniz
ed mice gave a significant specific proliferative response. IFN-gamma gene
expression was also detected after an ex-vivo specific stimulation of splee
n cells in all immunized mice. This response was independent of dose, H-2 g
enetic background or type of adjuvant. The results indicated that immunizat
ion with the Co.120 protein elicits a potent anti-HCV humoral and cellular
immune response. (C) 2001 Elsevier Science Ltd. All rights reserved.